Genetics and Society: Molecular Biology and Technology?

Question

example#1:If the DNA strand had the following sequence:
A C G C G T A G A A A C G C G G A T T A

then the enzyme that cuts it would be FnuD II beacuse it recognizes the sequence CGCG. Fnu II will cut the DNA between the C and the G. The cut strand of DNA would look like this:
A C G C G T A G A A A C G C G G A T T A

The result is 3 fragments of pieces of DNA. The first fragment contains 3 nitrogen bases, second frag contains 10 bases, and third contains 7 bases.

Determine which enzyme cuts each strand of DNA. Seperate each strand with a . where it should be cut. Indictae the name of enzyme, number of fragments produced, and size of each fragment(number of bases). You wont use all enzymes.

TABLE OF RESTRICTION ENZYMES
NAME ENZYME vs RECOGNITION SITE
Alu I AG<->CT
Bcl I T<->GATCA
Bal I TGG<->CCA
Cla I AT<->CGAT
FnuD II CG<->CG
Stu I AGG<->C
Mst II T<->CGA

DNA STRANDS:

1. T A A C G C G T C A T A A C G C G T T A A C G T T A A C G T C G C C T T

2. C C G T A T C G A A T C G A T A T G G C C A C T T A C G A

3. G C T A A T C G A T A T C G A T T G C A A C G T A C C T A

4. G G G C G A G C T C G T A T C A T T A G C T A C G A G A T T

5. T G G C C T A T C G A C G T G A T C A T A A C A G G C C C A

I need to know the enzyme, number of fragments, size of each fragment and where they should be cut.

Answer 1

ok you have plenty of statements but where is your actual question.

Answer 2

? Not trying to be funny but thisbit of DNA you've got here has, what, four codons maximum? That's four amino acids. An enzyme protein has a LOT more acid bases than four! If you need to try and work out what you may have on an 'average' humanDNA strand, try Sanger project at Cambridge or do a Datablast search. They may give you some possible answers.

Answer 3

Weeewwww.... You are coooolllll....

I know what u mean, but I think it is kinda wrong. Is that in the transcription process ??? If yes it is wrong. It is true that there is a spesific enzymes for a spesific bases site. But it is not strightly correct. That enzymes like u mention above just the 'non-used enzymes'
The true one is the DNA polymerase that bind into them and un wind the DNa strand.

And also like the above said, you are not mention the exact question.

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Answer 4

Apples and Oranges?

Answer 5

do your own homework!