For all of those that are well versed in biochemistry techniques I need some help in determining the ideal protocol for my research. So I am trying to determine a specific protease which degrades a receptor molecule that my lab studies but I am starting completely blank. So I thought that I should perform a DNA Microarray to determine gene expressio but I was wondering if anyone knows any other protocols which would allow me to view gene expression of many genes?
2006-07-16 09:47:32 · 2 answers · asked by js416256 1 in Science & Mathematics ➔ Biology
I am not sure I understand your problem.
Do you know the protease and you want to follow its expression or do you want to find which protease it is?
DNA chips are only good as a comparative study, ie you need to have a reference state-don't know what you are doing so I can't suggest anything.
There is also quantitative PCR and the proteomics path (2D-gels followed by Mass Spec Analysis) for determining expression levels.
If you don't know what's the protease, do you have any suspects?
Which organism are you working with? Are there any described homologues in other organisms which can guide you to certain proteases as candidates?
You don't give us enough info to help you out.
2006-07-16 10:21:52 · answer #1 · answered by bellerophon 6 · 0⤊ 0⤋
Microarrays are the premier way to look at gene expression if you know the function of a gene but not where it is or what its sequence is. Microarrays are designed for this purpose.
2006-07-16 10:13:55 · answer #2 · answered by DakkonA 3 · 0⤊ 0⤋