how is Blood sample analysed for heavy metals using AAS.kindly send me sample preparation of blood for AAS?

Question

SAMPLE PREPARATION OF BLOOD TO ANALYSE HEAVY METAS USING ATOMIC ABSORPTION SPECTRSCOPY

Answer 1

I work in a petroleum testing laboratory and we use an AAS on a daily basis. Although we don't test blood, I would assume you would either dilute a known weighted sample of blood in a blank solution (in my case we use a Kerosene/Xylene solution) and aspirate as normal and make our calculations from the absorbances of 3 standards and our test sample. The other way we run a sample on our AAS is to ash the sample. We burn it in a 750 degree celcius oven for approx 4 hours, after cooling, flux it with a 90/10 mixture of powdered chemicals (the names I cannot remember) in a 950 degree celcius oven for 10 minutes, boil the ash away with tartaric acid solution for 5 minutes, then make remaining solution aqueous by adding DI water up to a 100 mL flask. We would run that sample against 3 known standards and make our calculation from that.

Answer 2

No special preparation required.