on a gram stain is enterbacter cloacae a gram neg. rod?

Answer 1

Enterobacter cloacae is a gram-negative, rod-shaped bacterium that has peritrichous flagella, measures 0.3-0.6 x 0.8-2.0 µm, is oxidase-negative, catalase-positive, and is facultatively anaerobic. The bacterium is positive for Beta-galactosidase, arginine dihydrolase, ornithine decarboxylase, citrate utilization, nitrate reduction, and Voges-Proskauer reaction. Production of lysine decarboxylase, hydrogen sulfide, urease, tryptophan deaminase and indole are all negative. Acid is produced from many carbon sources. Reactions to many of these biochemical tests may be obtained using API 20E strips (Analytab Products). In addition, E. cloacae is negative for phenylalanine deaminase and pectate degradation.

Enterobacter cloacae grows well on standard bacteriological media on which yellow pigment and purple stain are not produced. Colonies are creamy tan on yeast extract-dextrose-calcium carbonate agar, and dark pink to burgundy with translucent margins on tetrazolium chloride agar. Miller-Schroth medium is useful for initial screenings because of the positive reaction (orange colonies) of E. cloacae on this medium.

Although this bacterium is part of the normal flora of the human intestinal tract, several species cause opportunistic infections of the urinary tract as well as other parts of the body. E. aerogenes and E. cloacae are two such pathogens that do not cause diarrhea, but that are sometimes associated with urinary tract and respiratory tract infections.

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Answer 2

Enterobacter cloacae is a gram negative bacillus. It is oxidase negative, catalase positive. Ferments glucose, and reduces nitrates to nitrites. They often cause bacteremia and are often mutidrug resistant. Antibiotic resistance cultures are highly advised.
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