what is the difference between PVP40and PVPP?

Question

when we use them in electrophoresis?

Answer 1

The chemical action of polyvinylpolypyrrolidone (PVPP; insoluble) and PVP (water soluble) in removal of inhibitors is based on the binding of phenolic groups. The formation of PVP-phenolic complexes removes molecules, such as humic acids, that contain phenolic groups. PVPP has been used to remove PCR inhibitors from Cryptosporidium-contaminated stool and soil samples.

PVP 40 is a lower-molecular-weight form of PVP containing fewer reactive sites and possessing greater solubility. The lower effectiveness of PVP 40 in blocking inhibition is likely due to the reduced capacity of PVP 40 to bind phenolic compounds in the samples.