What are some good practices in the laboratory ?

Question

Molecular lab

Answer 1

Straight from our lab safety agreement:

1.The laboratory is to be used for serious work. Only businesslike behavior will be tolerated.
2.Do not perform experiments that are unauthorized. Always obtain your instructor’s permission.
3.Study your laboratory assignment before you come to the lab. If you are in doubt about any procedure, ask your instructor for help.
4.Use the safety equipment provided for you. Know the location of the fire extinguisher, safety shower, fire blanket, and first aid kit.
5.Report any accident, injury, or incorrect procedure to your instructor at once.
6.Smother fires with a towel. If clothing should catch fire, smother it with a blanket or coat or quench it under a safety shower. NEVER RUN.
7.Handle toxic, combustible, or radioactive substances only under the direction of your instructor. If you spill acid or another corrosive chemical, wash it off immediately with water. Never taste any chemical substance or draw poisonous materials into a glass tube with your mouth. Keep combustible materials away from open flames.
8.Place broken glass and solid substances in designated containers. Keep insoluble waste material out of the sink.
9.Use electrical equipment only under the supervision of your instructor. Be sure your instructor checks electrical circuits before you activate them.
10.When your investigation is completed, be sure to turn off the water and gas and disconnect electrical connections. Clean your work area, Return all materials and apparatus to their proper places.
11.Safety goggles must be worn.
12. No food or drink in the laboratory. Many of the chemicals used are poisonous, and you would not want to accidently ingest them.

Answer 2

Six easy steps.

1) Before you walk through the door, always, always, always read the entirety of that day's plan. Do not ever walk into the lab without a general idea of materials needed and the order of the steps you will be following. You should also be able to recite, without thinking, the abstract and concrete reasons for the experiment. If you have designed your own experiment, do not skimp on cleanliness or detail. That is, make sure that your plan for that day is legible and easy to follow. Also ensure that your materials list is precise.

2) Once you get to your table, read the entirety of that day's plan. I know you have already read it. But, I cannot stress this enough.

3) Read the entire step, even step one, before carrying it out.

4) Record all measurements to the best of your ability. And make sure to record them as far as you can. This means recording as you work and does not mean trying to remember measurements a few minutes later. Also, you may need only two decimal places for the report, but having five in your lab book will help you, should you run into any problems.

5) I know this is cliche, but observe all safety requirements for your particular lab. Never horse around, even around safe experiments. Keep your working space as clean and clear of obstruction as possible. Do not eat or drink in the lab. Always wear your PPE (Personal Protection Equipment). Yadda, yadda.

6) Finally, do not ever stop an experiment unless you are absolutely sure that you have contaminated or otherwise fouled it. Your results may be different than those of your peers. That is fine. Confer with them later, to determine who needs to perform the experiment a second, or even fifth, time.

Answer 3

this isn't proper because you're utilising too a lot phenolphthalein. Phenolphthalein is itself an acid. In its acidic variety it has 3 fragrant earrings that are all conjugated independently of one yet another. The electrons can bypass around the earrings, yet no longer from ring to ring. at the same time as an acidic proton is plucked from between the exterior oxygens, you get some resonance. This strikes the bonds only sufficient that now you'll get conjugation for the time of all 3 earrings. The electrons can now bypass in the course of the completed molecule! This motives the molecule to soak up a a lot distinct spectrum of sunshine. therefore, a distinct colour. This procedure occurs round a pH of 8 or 9. even if, because pH graphs are exponential, it in undemanding words takes some drops of base to an acid answer to make this wide pH leap. because of this you bypass slowly close to the proper of a titration, the pH is shifting plenty! Like I suggested, the phenolphthalein is an acid itself. upload too a lot, and it is going to protonate the bottom itself! some drops is sufficient to work out the colour replace, and steer away from attending to on the point of a spontaneous neutralization. also, in the journey that your indicator is too a lot of an acid presence in answer, you'll ought to count number the ten mL as component of the 25 mL acid answer. You effectively have 35 mL of acid because of all the phenolphthalein. relaxing actuality: phenolphthalein is likewise a non-prescription laxative. that is off the marketplace even if, via the indisputable fact that is somewhat carcinogenic.

Answer 4

1. do not smell anything (at least not directly).
2. Do not taste anyhing
3. no makeup
4. take off your contacts if you wear them
5. no loose clothing
6. remember that the lab is not a social club and you will be fine!

Answer 5

depends on which type of lab you're in...chemistry, biology, genetics, molecular???

generally speaking...don't leave breakable things lying around, dispose of broken glass in proper receptacle, always wear gloves, keep environment sterile, know what chemicals you're using and how to clean them up if you spill them, know where safety equipment (fire blanket, eye wash, shower, fire extinguisher) is at all times....etc

Answer 6

1) Wash your hands
2) Wear tight fit gloves
3) Wash all apparatus with appropriate liquids before and after experiments

Answer 7

Don't taunt the test monkey.
Don't drink anything blue.
Don't drop the Ebola virus dish.

Answer 8

Apart from all routine good practices, only one good practice stand out.. that is... don't indulge in any malpractice !

Answer 9

Be careful and make sure you know exactly what you're doing.