what is the difference between C8 and C18 HPLC column?

Question

In RP-HPLC we r using no.of columns . in silica columns there are C4, C8,andC18 Columns.C8 is almost similar to C18 column. but in what way it differs?

Answer 1

Typically, all GC and HPLC columns are made of a silicaceous material, which is then coated on the interior surfaces with a variety of materials to modify their absorptive/attractive qualities. A C8 column has a variety of 8-carbon alkyl groups (could be n-octyl, or perhaps methyl-heptyl, etc) bonded to the silica surface; a C18 column has 18-carbon alkyl groups bonded; a C4 column has butyl-groups.

The differing nature of the treatments alters the attractive qualities of the column walls, thereby altering the retention times of the various analytes. If you have an idea what your analytes are, you can then chose the specific column that will do a better job of separating them.

Answer 2

C8 Column

Answer 3

are you attempting to be sure if a pattern has ethanol? if so you ought to use a familiar column with a refractive index detector. in case you utilize a UV detector, you will no longer even see an ethanol height. to tell the reality you will have the potential to smash out with a C18 column, yet this would be weird and wonderful, high priced, and counting on your solvent would no longer be stable for the column.

Answer 4

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