How do i isolate DNA?

Answer 1

Easiest method involves the pooling of the source (best source is wheat germ due to ease) next grind the germ then add some detergent solution- this will lyse the cell wall (the grinding breaks down the plant wall) next is to add some form of alcohol (isopropanol or high % ethanol is best (91% Isopropanol is available at drug stores)) Add the alcohol and the DNA will float to the top with the solution- spool on a glass rod.

Answer 2

There are various extraction techniques. The goal of DNA extraction is to make the DNA stable. Any raw solution of tissue will contain DNAases which will chew up DNA and you don't want that. The goal behind DNA extraction is to prevent them from doing that.

The first stage in extaction is breaking the cells apart and releasing their contents into solution.

Simple salt ethanol precipitation extraction techniques will cause the DNA to clump up and fall out of solution. The proteins (including DNAases) are removed along with the supernatant.

Other protocols such as phenol/chloroform will remove the proteins. In this case, the proteins will fall out of solution and the DNA is left dissolved.

Finally, other extractions such as chelex-based extractions will leave basically everything in solution. They work by binding up metal ions that are required to catalyze the DNAase reactions. The DNAases are there, they just can't do their job.

Answer 3

Promega has great products to do this easily.

www.promega.com They have free samples a lot.

Answer 4

Put it in a room by itself.

Answer 5

try this

http://en.wikipedia.org/wiki/DNA_extraction