生化專業原文..

Question

請幫我翻譯下列原文,如是用任何翻譯軟体,一律不給奌數..最好能用一般的話翻出來,不要翻的太文言文,因為要報告用的..感謝.

Protein synthesis in liposomes with a minimal set of enzymes

In a significant step towards the construction of the semi-synthetic minimal cell, a protein expression system with a minimal set of
pure and specific enzymes is required. A novel cell-free transcription and translation system named PURESYSTEM (PS), consisting
of a specified set of 36 enzymes and ribosomes, has been entrapped in POPC liposomes for protein synthesis. The PS has been used
to transcribe and translate an Enhanced Green Fluorescent Protein (EGFP) gene from plasmid DNA. The synthesis is confirmed by
the EGFP fluorescence emitting liposomes on fluorometric analysis and on confocal microscopy analysis. Furthermore the PS encapsulated
into POPC liposomes can drive the expression of the plsB and plsC genes encoding for the sn-glycerol-3-phosphate acyltransferase
(GPAT) and 1-acyl-sn-glycerol-3-phosphate acyltransferase (LPAAT) involved in the first step of the ‘‘salvage pathway’’ for synthesis of
POPC. The expression of GPAT and LPAAT in liposomes would in principle allow the production of the cell boundary from within.

= =.....都說了..不要用翻譯軟体...比我自己翻的还爛..

Answer 1

蛋白質綜合在liposomes 裡以最小的套酵素

在重大步往半合成最小的細胞的建築, 蛋白質表示系統以最小的套
純淨和具體酵素必需。一個新穎的無細胞副本和翻譯系統說出PURESYSTEM (PS 名字), 包括指定的套36 酵素並且核糖體, 被坑害了在POPC liposomes 裡為蛋白質綜合。PS 被使用了抄錄和翻譯一個改進的綠色螢光蛋白質(EGFP) 基因從質粒DNA 。綜合由EGFP 熒光證實散發liposomes 在熒光分析和在confocal 顯微學分析。此外PS 濃縮了入POPC liposomes 可能駕駛plsB 和plsC 基因的表示輸入為錫丙三醇3 磷酸鹽acyltransferase (GPAT) 並且1 醯錫丙三醇3 磷酸鹽acyltransferase (LPAAT) 被介入在"搶救路的" 第一步為POPC 綜合。GPAT 和LPAAT 表示在liposomes 裡原則上會允許細胞界限的生產從內。

Answer 2

原文:
Protein synthesis in liposomes with a minimal set of enzymes
In a significant step towards the construction of the semi-synthetic minimal cell, a protein expression system with a minimal set of pure and specific enzymes is required. A novel cell-free transcription and translation system named PURESYSTEM (PS), consisting of a specified set of 36 enzymes and ribosomes, has been entrapped in POPC liposomes for protein synthesis. The PS has been used to transcribe and translate an Enhanced Green Fluorescent Protein (EGFP) gene from plasmid DNA. The synthesis is confirmed by the EGFP fluorescence emitting liposomes on fluorometric analysis and on confocal microscopy analysis. Furthermore the PS encapsulated into POPC liposomes can drive the expression of the plsB and plsC genes encoding for the sn-glycerol-3-phosphate acyltransferase (GPAT) and 1-acyl-sn-glycerol-3-phosphate acyltransferase (LPAAT) involved in the first step of the ''salvage pathway'' for synthesis of POPC. The expression of GPAT and LPAAT in liposomes would in principle allow the production of the cell boundary from within.
譯文：
在最低酵素成份組合微脂粒中的蛋白質合成
在半合成最低組合成份細胞結構研究的1個重大進步中，含最低組合成份的純化特殊酵素的蛋白質表現系統即屬必需。即1個名為純化系統(PS)新型的無細胞成份的轉譯和轉化系統中，即含有36種酵素和核糖體，已嵌入POPC的微脂粒中，用於蛋白質的合成。PS已用於可轉譯的物質中，且自質體DNA轉化成增強型綠色熒光蛋白(EGFP)的基因。本合成作用可由螢光分析和共焦V中的EGFP螢光將微脂粒放射得到確認。再者，PS 壓縮封於POPC微脂粒中可促成plsB 和請求的基因表現，即為POPC合成作用第1個步驟中，用於甘油-3-磷酸轉??(GPAT) (sn-glycerol-3-phosphate acyltransferase)和溶血性磷脂酸?基轉移?(LPAAT) (1-acyl-sn-glycerol-3-phosphate acyltransferase)的編碼。
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