Turners Design Luminometer TD 20/20是什麼意思?
原文如下:用【 】框起來了
2.3. Transient transfections
Transfections in Hep3B cell cultures in 6-well plates were carried out using
the LipofectAMINE reagent (Qiagen, Velencia, CA) and the manufacturer's
protocol. Briefly, 250 ng of reporter constructs and 50 ng of RSV β-gal was used
in each experiment. Four hours later the medium was changed. After 24 h of
transfection, cells were treated for an additional 6 h with IFN-γ (10 ng/ml), IL-6
(10 ng/ml), and Dex (100 nM) alone or in combination as indicated in respective
figure legends. Whole cell extracts were prepared by extraction with 200 μl of
lysis buffer (Promega, Madison, USA). An aliquot of the cell extract was used tomeasure luciferase activity using a luciferase assay system (Promega) and
【Turners Design Luminometer TD 20/20 as described by the manufacturer.】
Luciferase activity was normalized with the β-gal activity. β-gal activity was
determined as described previously
實在是查不到!拜託了...
2006-10-27 21:58:05 · 1 個解答 · 發問者 小明 1 in 科學 ➔ 化學
Turners Design----->公司名稱---->他在美國
Luminometer ----->螢光光度計
TD 20/20 ----->型號
你框起來的英文應該是說螢光光度計的規格就如廠商說明書所示.而你問的Turners Design Luminometer 他就只是一台螢光光度計.用來分析螢光.
2006-10-27 23:49:15 · answer #1 · answered by Sai 6 · 0⤊ 0⤋